Discerning changes in multiple subpopulations is challenging, and subtle trends might be missed if traditional reference ranges derived from a control population are applied. Genetic primary immunodeficiency diseases are increasingly recognized, with pathogenic mutations changing the composition of circulating leukocyte subsets measured by flow cytometry (FCM). 5Department of Hematology, The Canberra Hospital, Canberra, ACT, Australia.4Department of Immunology, The Canberra Hospital, Canberra, ACT, Australia. 3Department of Nephrology, The Canberra Hospital, Canberra, ACT, Australia.2Centre for Personalised Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.1Department of Immunology and Infectious Diseases, Australian National University, Canberra, ACT, Australia.Jiang 1,2,3, Amelia Cook 1,2, Rochna Chand 1,2,4, Dipti Talaulikar 5, Ann-Maree Hatch 2, Anastasia Wilson 2, Carola G. Ellyard 1,2, Robert Tunningley 1,2, Ayla May Lorenzo 1,2, Simon H.
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